Development of a SYBR green I-based quantitative RT-PCR for Ross River virus: Application in vector competence studies and antiviral drug evaluation
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منابع مشابه
Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
متن کاملdevelopment of sybr green i based real-time rt-pcr assay for specific detection of watermelon silver mottle virus
background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...
متن کاملDevelopment of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus.
BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
متن کاملDevelopment and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus
BACKGROUND Avian hepatitis E virus (HEV) is the main causative agent of big liver and spleen disease, as well as hepatitis-splenomegaly syndrome in chickens. To date, conventional reverse transcriptase polymerase chain reaction (RT-PCR) and nested RT-PCR methods have been used for the diagnosis of avian HEV infection in chickens. However, these assays are time consuming, inconvenient, and canno...
متن کاملRapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR.
INTRODUCTION Dengue fever and dengue haemorrhagic fever currently rank highly among the newly-emerging infectious diseases, and are considered to be the most important arboviral disease worldwide. The definitive diagnosis is culture analysis, but practical considerations limit its use. Also, the period for viral detection is limited. Within a day or two after fever subsides, rising levels of an...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2016
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2016.04.011